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publicationDate 2000-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1009842-A1
titleOfInvention Plasmids for plant transformation and method for using the same
abstract The invention provides novel Ti-plasmid vectors for Agrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that reduces or eliminates the occurrence of transformants with vector read-through DNA sequence. The invention also provides methods of plant cell transformation utilizing these novel vectors.
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2248902-A2
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