| abstract |
The present invention provides a simple method for thendetermination of a specific component, e.g. 1,5-anhydroglucitoln(1,5-AG) in a sample containing glucose, andna reagent and a reagent kit useful in the method. In onenembodiment, a method for the determination of 1, 5-AG is providednwhich comprises contacting the sample with an enzyme systemnwhich converts glucose into fructose-1,6-diphosphate andnconverts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate,ndehydrogenating 1,5-AG-6-phosphate in the sample bynthe action of 1,5-AG-6-phosphate dehydrogenase in the presencenof an oxidized coenzyme, and measuring the amount of the reducedncoenzyme formed by the dehydrogenation reaction. A reagent andna reagent kit useful in this method are also provided. |