http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1006180-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_593f1a3ba6ae19dde0c7ddf687e26640 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4d5d546aa6cbfb52c137af7cafc36bf4 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 1999-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24769328a2849756300e16f32a79e532 |
publicationDate | 2000-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1006180-A1 |
titleOfInvention | Method for producing double stranded DNA whose terminal homopolymer part ist eliminated and method for determining nucleotide sequence |
abstract | Disclosed is a method for producing double-stranded DNAncomprising treating double-stranded DNA having a homopolymernpart or parts at one or both ends with a restriction enzymento partly or fully eliminate at least one of the homopolymernpart or parts. The restriction enzyme is capable of cleavingndouble-stranded DNA at a cleavage site separate from anrecognition site therefor. Disclosed is a method forndetermining a nucleotide sequence of double-stranded DNAnutilizing one or both strands of the double-stranded DNA asna template, wherein the double-stranded DNA used as thentemplate is double-stranded DNA prepared by the above methodnof the present invention. The present invention provides anmethod for producing double-stranded DNA a part or all of whichnhomopolymer part, which may inhibit nucleotide sequencendetermination, is eliminated, and a method for determining annucleotide sequence utilizing as a template the double-strandednDNA prepared by the above method of the presentninvention, whose homopolymer part is partially or fullyneliminated. |
priorityDate | 1998-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.