| abstract |
The invention provides a process for preparing an opticallynactive 3-hydroxypyrrolidine, wherein an oxygen atom isninserted stereoselectively into the corresponding pyrrolidinenby use of a biocatalyst. The biocatalyst may be selected fromnthe group consisting of alkane hydroxylases, alkanes-degradingnstrains, mono-alicyclic hydrocarbons-degradingnstrains, microorganisms or enzymes from the generan Pseudomonas , Aspergillus , Mycobacterium , Corynebacterium ,n Nocardia , Gordona , Beauveria , Fusarium , Bacillus ,n Streptomyces , Rhizopus , Candida , Rhodococcus , Methylococcus ,n Sebekia , Penicillium , Mortierella , Gibbeerella ,n Scopuloriopsis , Curvularia , Gnomonia , Mucor , Wojnowicia ,n Ophiobolus , Deadalea , Leptosporus , Diaporthe , Colletotrichum ,n Absidia , Syncephalastrum , Phycomyces , Botryosphaeria , andn Nigrospora . |