| abstract |
A method of immobilizing aminated DNA to a substrate surface is provided for use in DNA hybridization assays. The surface can be widely employed in a variety of molded laboratory products including multiwell plates, cell culture dishes, and biological containers. The method comprises the steps of providing a polystyrene substrate having a surface content of between 0.3 and 4.0 % atomic percent oxygen, and attaching amine modified oligonucleotide to the surface of the substrate. A resultant product comprising a polystrene substrate having a surface oxygen content of between 0.3 and 4.0 % atomic percent, and a plurality of amine modified oligonucleotides directly attached to the surface of the substrate, is also disclosed. |