| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f3fd1afe3264d1076b11f3f63e282359 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2428 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-34 |
| filingDate |
1997-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_acd5395f10269a04a4e7bc77c204e76d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c5f1e3d5444d2eba33d7f19189eefc3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1a1cfcbc76a9f5c3dbc12a63b9dab3c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fa111eb9fd371d143e82f2282ce2591
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63fe50789fc3a47cee1256ff14ca339f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b122f4424034067144cad93d0ea8fb0d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a41ef86cb0243dcad2ef2b0d3cf42cd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7aeb6d8e2a55ca59eb7695de9347676a |
| publicationDate |
2000-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0970193-A1 |
| titleOfInvention |
PROTEIN ENGINEERING OF GLUCOAMYLASE TO INCREASE pH OPTIMUM, SUBSTRATE SPECIFICITY AND THERMOSTABILITY |
| abstract |
A fungal glucoamylase including a mutation pair Asn20Cys coupled with Ala27Cys forming a disulfide bond between the two members of the pair. The mutation provides increased thermal stability and reduced isomaltose formation to the enzyme. A fungal glucoamylase including a 311-314Loop mutation, wherein reduced isomaltose formation is provided by the mutation, is also provided. A fungal glucoamylase, including a mutation Ser411Ala wherein increased pH optimum and reduced isomaltose formation is provided by the mutation, is also provided. Combinations of the mutations in engineered glucoamylases are also provided as are combinations with other glucoamylase mutations that provide increased thermal stability, increased pH optimum and reduced isomaltose formation for cumulative improvements in the engineered glucoamylases. |
| priorityDate |
1996-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |