| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c163c101399a77181408b93dc02fe00d |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P35-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0012
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0024 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
| filingDate |
1998-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf6be9b2efc77d97a983d9c1a1bf693b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1db65ca73a95c139b80c4d5e1bd2646
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_abf2b331e3bee431e9c2e9401eef7837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a45d18926a0d940f94fe32e800a9609a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_72de562331f70c7d73d62cee6c29ebe0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9127bf0e06172e04d49e005a07b6ae2c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e01fcc7a1cc036752dec984d6b2b39ff
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e8e280965ef2844a4595dfc860ca6c8 |
| publicationDate |
2000-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0969088-A1 |
| titleOfInvention |
ENZYMATIC PROCESS FOR THE PREPARATION OF CEPHALOSPORANIC 7$b(g)-(4-CARBOXYBUTANAMIDE) ACID BY MEANS OF THE MODIFIED ENZYME D-AMINOACID OXIDASE OF $i(TRIGONOPSIS VARIABILIS) PRODUCED IN $i(ESCHERICHIA COLI) |
| abstract |
Enzymatic process for the preparation of cephalosporanic 7β-(4-carboxybutanamide) acid bynusing the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichiancoli . The process for the expression of the enzyme comprises: (I) isolating the DNA correspondingnto the gene which codes for the enzyme D-aminoacide oxidase; (II) removing the intron which isncontained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capablenof replication in Escherichia coli ; (IV) fusing at the extremity 5' of the structural region of thengene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V)ntransforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating thentransformed cells of Escherichia coli ; and (VII) recovering the enzyme D-aminoacid oxidase of thenformer cultivation operation through affinity chromatography. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1748073-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1925663-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7517677-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1925663-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1748073-A1 |
| priorityDate |
1997-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |