| abstract |
The live recombinant avian vaccine comprises, as vector, an ILTV containing and expressing at least a heterologous nucleotide sequence, said nucleotide sequence being inserted in the insertion locus formed by the internal ribosome entry site (IRES) located between the stop codons of COL D and COL E of ILTV and which, in a particular ILTV strain is defined between nucleotides 3873 and 4260 at SEQ ID NO:1. The heterologous nucleotide sequence can be under the control of a strong eukaryotic promoter, such as CMV-IE promoter, and can be derived from viruses of Newcastle disease, Marek disease, Gumboro disease, infectious bronchitis, chicken anaemia, chicken pneumovirosis. The invention also concerns a multivalent vaccine comprising at least two live vaccines as per the invention and the recombined ILTV virus. |