| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3244ba8390b1f290f4ba36389c82488b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-002 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-505 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q-
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K-
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-505
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N- |
| filingDate |
1998-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f737e466d0142bf2b051b74182c6fec5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c218274af4acd35eb0a56936e02373b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27ce560714137f397930728d2cf88dd8 |
| publicationDate |
1999-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0957165-A2 |
| titleOfInvention |
Optimizing cells for endogenous gene activation |
| abstract |
The invention relates to methods for optimizing gene expression in cells. A first aspect relates to a method for changing the expression of a target gene endogenously present in a eukaryotic cell by introducing a heterologous expression control sequence into the genome of the cell by means of homologous recombination, and the cutting out of the inserted foreign DNA mediated by a site-specific recombinase and its replacement by others heterologous expression control sequences and / or amplification genes. The invention further relates to the introduction of one or more nucleic acid sequences to which an activator protein or an activator protein complex, for example a hypoxia inducible factor (HIF) binds, into the genome of a eukaryotic cell by homologous recombination in order to change the expression of a target gene. Furthermore, the invention relates to a method for testing the influence of 5'-sided or 3'-sided non-coding nucleic acid fragments on the expression of a target gene by determining the expression of a reporter gene. The invention also relates to a method for providing a DHFR-negative eukaryotic cell containing a recombinase target sequence and the expression of a nucleic acid sequence inserted in the recombinase target sequence. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9752140-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8409861-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9695442-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9289451-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0166717-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9782437-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8106255-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9145565-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8524500-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9447434-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7888121-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7972854-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9249428-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10675302-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10669557-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8349810-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11311574-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0166717-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10023887-A1 |
| priorityDate |
1997-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |