| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d4c34a5b7ef6627a66f56ac05f5535a |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2431
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01026 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-72 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 |
| filingDate |
1997-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee82fbee4ffc25d1a76d306fe3c7a39e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_029753e1203f79a87ca36d7eaf33891d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c7df8dacf7423697ef0641ea72aa199
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e34087cb849fbba9023ea29cb2a6362
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb7cde9cc7e51727bdbd2dc4e3fe8729
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f8d8a278ca7172045920bbd9fefb45a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0e1b92067f32c5fbca3729f4b911e05 |
| publicationDate |
1999-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0956356-A1 |
| titleOfInvention |
$i(CANDIDA UTILIS) TRANSFORMATION SYSTEM |
| abstract |
The present invention discloses a transformation system useful to express heterologous proteins in the Candida utilis yeast, based on obtaining auxotrophic mutants of said species as well as the isolation of different genes, from a genomic library, which complement said auxotrophies. The transformation system uses as hosts new auxotrophic mutants obtained from the yeast NRRL Y-1084 of Candida utilis which are defective mainly in the biosynthetic ways of uracyl and histidine, which are transformed with plasmids containing as selection markers the genes URA3 and HIS3 of Candida utilis. Another aspect of the invention is the isolation of the gene coding for the enzyme sucrose invertase or beta -fructofuranosidase of Candida utilis, as well as the identification of sequences for promoting, secretion signalling and termination of said gene INV1. These sequences are useful to obtain the expression of heterologous proteins in said yeast. |
| priorityDate |
1996-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |