http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0948649-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3578a066fa047e9699ef7e54ee66620b |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1075 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 1997-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c50cda525cbf087fc152d4f588876030 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6daaad177fd86fb91ddefcc062dc1d58 |
publicationDate | 1999-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0948649-A1 |
titleOfInvention | Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom |
abstract | The present invention relates to a method for the comparative assessment of the level of specific nucleic acid sequences in samples derived from different sources. More specifically, the invention relates to a method using oligonucleotides covalently linked to a solid support, such as beads, to isolate specific labeled nucleic acid sequences from complex mixtures. The methods disclosed allow quantitative comparisons of the amount of nucleic acid of defined sequence in a plurality of different samples of nucleic acid, e.g., from different cells or tissues or from genetic libraries. Nucleic acids from the samples are labeled in such a fashion that the signals can be distinguished and compared following hybridization to the oligonucleotides on the beads. According to the invention, the solid supports with the hybridized nucleic acid may be retrieved, and the target nucleic acid eluted and analyzed. Furthermore, the invention provides a method for tagging individual clones from a cDNA library such that they can be identified uniquely and retrieved by hybridization to specific beads. |
priorityDate | 1996-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 225.