Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0cf3371a9dd0e2fab14e900377fd1096 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D219-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D219-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D219-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09B15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D219-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D219-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D219-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09B15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
filingDate |
1997-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94e5308f023e747946cd8506c3e17ead http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e946f5060d99433071e2c4b12e8bcdc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41a2027a8dafaca26ed3e3806537bfbd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b74b443cc5141cd3f639e2ee32d94b56 |
publicationDate |
1999-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-0938677-A1 |
titleOfInvention |
Chemiluminescent detection methods using dual enzyme-labeled binding partners |
abstract |
Methods of detecting analytes or target species using two enzyme-labeled specific binding partners where the two enzymes function in concert to produce a detectable chemiluminescent signal are disclosed. The methods use a specific binding partner labeled with a hydrolytic enzyme to produce a phenolic enhancer in close proximity to a peroxidase-labeled second specific binding partner. The method is useful to detect and quantitate with improved specificity various biological molecules including antigens and antibodies by the technique of immunoassay, proteins by Western blotting, DNA by Southern blotting, RNA by Northern blotting. The method may also be used to detect DNA mutations and juxtaposed gene segments in chromosomal translocations and particularly to unambiguously identify heterozygous genotypes in a single test. |
priorityDate |
1996-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |