http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0938299-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d39b4893e9805c511f9d0d1a55c19d1c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-1658 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-5169 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-5192 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K49-223 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-51 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K49-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-16 |
| filingDate | 1997-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd3186a76d271be3b0ee8a92b5cd8e55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_190411bb172c04f2ea36750aed930088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_501f8795b74db2cccf1700848ac695f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b11417891450891ffbf70e19deef40b8 |
| publicationDate | 1999-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0938299-A1 |
| titleOfInvention | Methods for the production of protein particles useful for delivery of pharmacological agents |
| abstract | A method has been developed for the formation of submicron particles (nanoparticles) by heat-denaturation of proteins (such as human serum albumin) in the presence of multivalent ions (such as calcium). Also provided are novel products produced by the invention method. An appropriate concentration of multivalent ions, within a relatively narrow range of concentrations, induces the precipitation of protein in the form of colloidal particles, at a temperature which is well below the heat denaturation temperature of the protein (as low as 60 °C for serum albumin). Temperatures at which invention method operates are sufficiently low to permit incorporation of other molecules (e.g., by co-precipitation), into submicron particles according to the invention, including compounds which cannot withstand high temperatures. Invention methods facilitate the production of protein nanoparticles and microparticles containing various molecules (such as nucleic acids, oligonucleotides, polynucleotides, DNA, RNA, polysaccharides, ribozymes, pharmacologically active compounds, and the like) useful for therapeutic, diagnostic and other purposes. The addition of multivalent cations serves both to induce precipitation, and to allow linking of negatively charged molecules, such as DNA, to the negatively charged protein. |
| priorityDate | 1996-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 866.