Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3120ae9cab5bb4831f0cc16288966367 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-38645 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D21B1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D21C5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D06M16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D06L4-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12S11-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12S99-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D21H21-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C11D3-386 |
filingDate |
1997-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16a85c45dcbad823e47f83b075db9638 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0baa5fb2f827da5ffc697dd857e7c19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e8c1f582050d4da49b68d80e70b6b61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28ce26ed4aed3dc63ee0ff074ddfd09a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b6dd88d891a93fa55638962fa66632d |
publicationDate |
1999-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-0937138-A1 |
titleOfInvention |
Cellulase variants |
abstract |
A method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: na. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; nb. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; nc. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5Å; nd. identifying surface-exposed amino acid residues of the enzyme; ne. identifying all charged or potentially charged amino acid residue positions of the enzyme; nf. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; ng. carrying out the substition, deletion or insertion by using conventional protein engineering techniques; and cellulase variants obtained by the method. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11497671-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8394237-B2 |
priorityDate |
1996-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |