http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0926235-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d913bbc990f78742487267dda0639e89 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6427 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-76 |
| filingDate | 1998-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a53c51ba978dad85e9f7d9769840beec |
| publicationDate | 1999-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0926235-A2 |
| titleOfInvention | Method of stabilizing trypsin |
| abstract | The present invention provides a method for stabilizing trypsin, in whichnenzyme reaction of trypsin can be generated in a two-solution system,ndegradation of trypsin and its substrate can be prevented, and enzymaticnactivity of trypsin is improved compared to conventional methods, and whichncan be sufficiently applied to an automatic analyzer. An enzyme solution isnprepared by dissolving trypsin in a buffer solution having a pH at whichnenzymatic activity of trypsin is active and containing calcium and/ornmanganese ions. It is preferable that the total concentration of the calciumnions and the manganese ions in the buffer solution is in the range of 3 to 10nmmol/l. It is also preferable that the concentration of the buffer solution is atnleast 10 mmol/l, and that the pKa of the buffer solution is higher than the pHnof the buffer solution. |
| priorityDate | 1997-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.