| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78463eaa98427d92d940df6de6842216
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c7ad58d3c0b071069a8ee0cb8c8ba2e8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24334
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24322
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-14143 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P15-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-185
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-187
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate |
1997-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
1999-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0924298-A1 |
| titleOfInvention |
Protein expression in baculovirus vector expression systems |
| abstract |
The invention relates to a method to increase proteinnexpression in baculo vector virus expression systems. Theninvention provides a method to produce a recombinant proteinnin insect-cell culture which comprises selecting anrecombinant baculovirus expressing said protein, growingninsect cells in growth medium in a culture vessel with ansufficient volume to contain at least 2 liters and infectingnthe cells with an inoculum of at least one baculovirus at ancell density of 1 x 10 5 to 5 x 10 6 cells/ml with an m.o.i ofn<0.01. n The invention furthermore provides a method to producenrecombinant pestivirus E2 or E rns protein or fragmentsnthereof in insect cell culture characterised by a finalnconcentration of said protein (fragments) in the growth mediumnat harvest of at least 100 µg/ml. n The invention furthermore provides a method to producenrecombinant follicle stimulating hormone, α-units and/ornβ-units and complexes and fragments thereof, at anconcentration in the growth medium at harvest of at leastn15 µg/ml. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101152570-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109182380-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0220048-A1 |
| priorityDate |
1997-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |