http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0917568-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6b2191eb50129bd29d6762bdcdf34e2d |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 |
filingDate | 1996-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ee98fa5b11abcab8a8d22002e6f4de5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a8e74254ddaf21b700c7d8d935802a46 |
publicationDate | 1999-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0917568-A1 |
titleOfInvention | Method for producing complex multienzymatical, storage resistant reaction mixtures and use thereof |
abstract | The invention describes a method and its use for producing complex multienzymatical, storage resistant reaction mixtures for synthesising, modifying or analysing polypeptides and optionally nucleic acids characterised in that native or artificial enzymatical, active protein mixtures with reaction buffers, cofactors and substrates are prepared so that they are ready for use and are storage resistant such that only user-specific key components (e.g. mRNS) are missing to start the desired enzymatical reaction (s). In the method a stabiliser is added to the reaction mixtures in the solution which, on the one hand, increases the reacting capacity of the multienzymatical systems and, on the other hand, protects the unstable reaction components from losing their biological activity or their biologically active structure whilst being made storage resistant and during storage. The reaction mixture is made storage resistant by being easily freeze dried under a vacuum and then durably stored at 4-10 DEG C (refrigerator temperature). Before use the user has to simply reconstitute the ready prepared reaction mixture by adding the original volume of H2O and start the desired enzymatical reaction(s) by adding the user-specific component(s). |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9738923-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11098344-B2 |
priorityDate | 1996-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 230.