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filingDate 1998-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c82cc9f7156e7e3a51d0520e423fadf1
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publicationDate 2001-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0902091-A3
titleOfInvention Method and kit for measuring the concentration or the activity of a protease inhibitor
abstract A method for measuring the concentration or the activity of UTInquickly and easily at high sensitivity. A urine sample, a buffer solution, antrypsin solution and a substrate solution are mixed and the trypsin activitynis then measured. Thus, the UTI concentration in the urine sample isndetermined. In this case, a substrate solution having only L-BAPNA is usednas the substrate, and the surfactant is mixed in at least one selected from thenbuffer solution and the enzyme solution. The mixing ratio of the surfactantnis about 1 wt.% in the entire enzyme reaction solution. Examples of thensurfactant include polyoxyethylene (40) octylphenylether, polyoxyethylenen(10) octylphenylether, 3-[(3-cholamido propyl) dimethylammonio]propanesulfonicnacid, 3-[(3-cholamido propyl) dimethylammonio]-2-hydroxypropanesulfonic acid, and polyoxyethylene sorbitan monolaurate.nAs shown in FIG. 1, the sensitivity improves when using L-BAPNA.
priorityDate 1997-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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