abstract |
The recombinant expression of human BMP2, BMP4 - BMP7 in murine mesenchymalnC3H10T½ progenitors mediates differentiation into three mesenchymal Iineages in differentnefficiencies: the osteogenic, the chondrogenic and the adipogenic lineage. This developmentaln in vitro model was used to identify and to characterize cDNAs involved during thenmanifestation of these lineages in vivo . By subtractive cloning an as yet undescribed cDNA, 29A nhas been cloned which encodes a putative secreted factor which is expressed in developingnosteo- /chondrogenic tissues of vertebrae, ribs, tooth and the limb bud. |