Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3120ae9cab5bb4831f0cc16288966367 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-185 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate |
1997-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2cd64075ab38961869ad14ad92650e65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a402633cf6b097bf29ed632b192fedf2 |
publicationDate |
1998-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-0873398-A1 |
titleOfInvention |
A method for in vivo production of a mutant library in cells |
abstract |
A method for in vivo production of a library in cells comprising a multitude of mutated genetic elements, wherein an error-prone polymerase is used in each ancestral cell to replicate all or a part of a genetic element independently of the host chromosomal replication machinery. The genetic element comprises i) an origin of replication from which replication is initiated, ii) optionally a genetic marker, e.g. a gene conferring resistance towards an antibiotic, iii) a gene encoding the polypeptide of interest. Also methods for the generation of a DNA sequence encoding a desired variant of a polypeptide of interest, and for the determination of such a DNA sequence are described. |
priorityDate |
1996-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |