http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0871780-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1386e6102cf5f941c15171928dd3ad7f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1997-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fe32c71955f7413e5668d7e9065e41a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eba8fa6f9fef6c97782198a76ee4b58f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d1fe89e655d83a76faf162abe86eab0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81e2482861c6fc6708863ccf12aca547 |
| publicationDate | 1998-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0871780-A2 |
| titleOfInvention | METHODS AND COMPOSITIONS FOR FULL-LENGTH cDNA CLONING |
| abstract | The present invention pertains to methods for the synthesis and cloning of full-length cDNA, or cDNA fragments, that correspond to he complete sequence of 5'-ends of mRNA molecules. The method of the present invention comprises contacting RNA with a cDNA synthesis primer which can anneal to RNA, a suitable enzyme which possesses reverse transcriptase activity, and a template switching oligonucleotide under conditions sufficient to permit the template-dependent extension of the primer to generate an mRNA-cDNA hybrid. The template switching oligonucleotide hybridizes to the CAP site at the 5'-end of the RNA molecule and serves as a short, extended template for CAP-dependent extension of the 3'-end of the ss cDNA that is complementary to the template switching oligonucleotide. The resulting full-length ss cDNA includes the complete 5'-end of the RNA molecule as well as the sequence complementary to the template switching oligonucleotide, which can then serve as a universal priming site in subsequent amplification of the cDNA. The subject invention also pertains to the template switching oligonucleotides that can be used according to the subject method. Kits containing the template switching oligonucleotide are also included within the scope of the present invention. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10266894-B2 |
| priorityDate | 1996-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 288.