| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12a135a933620c99beab01d4ac785d25 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2445
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2414
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2408
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01021 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H15-203
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H15-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H17-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H17-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H17-075
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 |
| filingDate |
1996-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eded5dfdd9707af7737daa66a72900ed
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_725db65e5902f2606bc957d0e2271c34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19b737293362692bd4d0e9f3edb5ed98 |
| publicationDate |
1998-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0870037-A2 |
| titleOfInvention |
Methods and compositions for synthesis of oligosaccharides using mutant glycosidase enzymes |
| abstract |
Mutant glycosidase enzymes are formed in which the normal nucleophilic amino acid within the active site has been changed to a non-nucleophilic amino acid. These enzymes cannot hydrolyze disaccharide products, but can still form them. Using this enzyme, oligoeaccharides are synthesized by preparing a mixture of an alpha -glycosol fluoride and a glycoside acceptor molecule; enzymatically coupling the alpha -glycosol fluoride to the glycoside acceptor molecule to form a glycosol glycoside product using the mutant glycosidase enzyme; and recovering the glycosyl product. The enzymes to be used are mutant forms of human or porcine alpha -amylase or yeast alpha -glucosidose having two catalytically active amino acids with carboxylic acid side claims within the active site, in the wild-type enzyme, with one of these amino acids being replaced by a different amino acid. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8143049-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8486683-B2 |
| priorityDate |
1995-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |