http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0859062-A3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9cb16f3933087407282046d3265c0c4e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-81 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-76 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
| filingDate | 1990-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56a332fa6635c36ab0fd2687a7909c81 |
| publicationDate | 2006-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0859062-A3 |
| titleOfInvention | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes |
| abstract | In an assay method in which a member of a specific binding pair is detected by means of an optically detectable reaction, the improvement wherein the optically detectable reaction includes the reaction, with an enzyme, of a dioxetane having the formulan nwhere T is a cycloalkyl or polycycloalkyl group bonded to the 4-membered ring portion of the dioxetane by a spiro linkage; Y is a fluorescent chromophore; X is hydrogen, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl, cycloheteroalkyl, or enzyme-cleavable group; and Z is hydrogen or an enzyme-cleavable group, provided that at least one of X or Z must be an enzyme-cleavable group, so that the enzyme cleaves the enzyme-cleavable group from the dioxetane to form a negatively charged substituent bonded to the dioxetane, the negatively charged substituent causing the dioxetane to decompose to form a luminescent substance that includes group Y of said dioxetane. |
| priorityDate | 1989-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.