| abstract |
A method for producing nucleoside-5'-phosphate esterninvolves phosphorylating nucleoside biochemically. n Nucleoside-5'-phosphate ester is produced bynallowing an acid phosphatase, especially an acidnphosphatase having an increased affinity for a nucleosidenand/or an increased temperature stability, to act at pHn3.0 to 5.5 on a nucleoside and a phosphate group donor.nThe phosphate group donor may be selected fromnpolyphosphoric acid or a salt thereof, phenylphosphoricnacid or a salt thereof, and carbamyl phosphate or a saltnthereof. The resulting nucleoside-5'-phosphate ester maynsubsequently be collected. As an alternative to usingnisolated acid phosphatase to catalyse the reaction, anmicroorganism transformed with a gene encoding a proteinnhaving acid phosphatase activity may be employed. |