http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0854195-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f97f0fa258a008bf056ad04b7f380dda |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1997-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94b937a257975a29f2c303f4b13977db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb49ad09e28fee2dc881058e5e035c23 |
| publicationDate | 1998-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0854195-A1 |
| titleOfInvention | Method for the amplification of nucleic acids in a salivary sample |
| abstract | The present invention provides a method of nucleic acidnsynthesis capable of directly amplifying a gene of interestnin a salivary sample without purifying DNAs from the sample. n According to the present invention, in a method ofnnucleic acid synthesis by mixing a salivary sample itselfnand a gene amplification reaction solution, and thennsubjecting them to an amplification reaction, the salivarynsample is heat-treated or treated with a saccharide-degradingnenzyme before the reaction. For example, ansalivary sample was heat-treated at 0-40 °C for 1 hour,nsubsequently added directly into a PCR reaction solution,nand then subjected to a PCR. The result demonstrates that,nas shown in Fig.3, the amount of the PCR product increasednaccording as the heat-treatment temperature was elevated,nand as a consequence, the PCR product sufficient to bendetected in the electrophoresis was produced in each of allncases using different amounts of saliva, by heat-treatingnthe sample at 30°C or above. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20020047693-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1464699-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1464699-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7312053-B2 |
| priorityDate | 1996-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1060.