| abstract |
The method serves for the screening of molecules from molecule libraries with regard to their individual binding behavior to at least one predetermined ligand. For this purpose, the ligands labeled with a fluorescent dye are mixed with the molecular library in the form of a suspension. After the excess, unbound ligands have been washed out, the mixture is plated onto a two-dimensional substrate (2). The local fluorescence intensities on the substrate are then recorded electro-optically in a fluorescence microscope (5) and electronically discriminated according to predetermined selection criteria. The objects selected and localized in this way are then sequentially positioned precisely by a coordinate-controlled displacement between the substrate (2) and a separation actuator (20, 21) and locally separated from the substrate (2) by the separation actuator (20, 21). |