http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0818460-A3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5c24684033fba97d1ad845ce453a57e3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-55 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-6561 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-10 |
| filingDate | 1997-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2996dd4fcc77ea56fbcd9f6b0350e982 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e7d2e61aa6eb02e2f86922c39f71c32 |
| publicationDate | 1999-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0818460-A3 |
| titleOfInvention | Synthesis of Oligonucleotides on a solid support. |
| abstract | a) sequentiellen Aufbau der Nucleotide an einem festen Träger nachnbekannten Methoden, wobei vorhandene exocyclische Aminogruppen annden Nucleobasen durch eine cyclische Diacylgruppe geschützt sind undngegebenenfalls vorhandene Phosphatschutzgruppen durch starke, nichtnnukleophile Basen abspaltbar sind, b) Entschützung der am festen Träger gebundenen Oligonukleotide, und c) Abspaltung der entschützten Oligonukleotide vom festen Träger nachnbekannten Methoden, ndadurch gekennzeichnet, daß die am festen Träger gebundenen Oligonukleotidenin Gegenwart einer starken, nicht nukleophilen Base in einem geeignetennorganischen Lösungsmittel entschützt werden.The invention therefore relates to a method for producing oligonucleotides by means of solid phase synthesis a) sequential assembly of the nucleotides on a solid support according to known methods, the exocyclic amino groups on the nucleobases being protected by a cyclic diacyl group and any phosphate protecting groups present being able to be split off by strong, non-nucleophilic bases, b) deprotection of the oligonucleotides bound to the solid support, and c) cleavage of the deprotected oligonucleotides from the solid support by known methods, characterized in that the oligonucleotides bound to the solid support are deprotected in the presence of a strong, non-nucleophilic base in a suitable organic solvent. |
| priorityDate | 1996-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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