| abstract |
A process for the fermentative production of lipstatin, which processncomprisesn a) aerobically cultivating a micro-organism of the order of actinomycetesnwhich produces lipstatin, in an aqueous culture medium which isnsubstantially free of fats and oils, and which contains suitable carbon andnnitrogen sources and inorganic salts, until the initial growth phase isnsubstantially finished and sufficient cell mass has been produced, and b) adding to the broth linoleic acid, optionally together with caprylic acid,n[wherein part or the totality of the linoleic acid and/or of the caprylic acid cannbe replaced by the corresponding ester(s) and/or salt(s)], and N-formyl-L-leucinenor preferably L-leucine, the linoleic acid or its ester(s) or salt(s) beingnstabilized by an antioxidant. n Lipstatin can be isolated from the broth and hydrogenated to tetrahydrolipstatin. |