| abstract |
The invention relates to a dye-labeled antibody conjugate comprising an antibody conjugate and a cyanine dye. The dye-labeled antibody conjugate is prepared by polymerizing an antibody such as mouse IgG in phosphate buffer using a polyfunctional reagent such as dithiobis(sulfosuccinimidylpropionate), and stirring the antibody and a cyanine dye represented by Formula I. The dye-labeled antibody conjugate is more sensitive to antigens than conventional dye-labeled antibodies because it has many reactive sites to antigens.n wherein X represents a halogen, M represents a hydrogen or alkali metal, and n represents an integer of 1 to 4 |