| abstract |
PCT No. PCT/GB95/02763 Sec. 371 Date Jul. 22, 1997 Sec. 102(e) Date Jul. 22, 1997 PCT Filed Nov. 27, 1995 PCT Pub. No. WO96/16733 PCT Pub. Date Jun. 6, 1996A method for treating silica to render its surface non-polar, especially for use as a chromatography medium in "reverse phase" chromatography. The silica is treated with a solution of a complex in which a hydrocarbon chain is coordinated, e.g. through a carboxylic group, with a metal ion such as chromium. The complex also has halide ligands, which on hydrolysis give rise to cationic charging and hydroxyl-bridging of the complex molecules with the silica surface and with one another. Elimination of water binds the molecules onto the silica surface where their hydrocarbon chains give the desired non-polar properties. |