Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69998c2799e158b092ade27db887f5c1 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0676 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-39 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-39 |
filingDate |
1995-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_972fab99c0141183193b45d3d4efe736 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93f09e12606570676635ba698bce6d3b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af02ec3f3e62bea28cbc54f9bf7ba973 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a571ca61808f01d4a5f969f1aae8344e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_707b1455b7ccad1651cb33f3bb0d6eea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4859d13dcf8868cf8a195bb026100c29 |
publicationDate |
1997-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-0766733-A1 |
titleOfInvention |
A purified mixture of collagenases and two other proteases obtained from clostridium histolyticum |
abstract |
A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cells clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters. |
priorityDate |
1994-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |