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filingDate 1994-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1999-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0763133-A4
titleOfInvention METHOD FOR DETECTION OF A TARGET NUCLEIC ACID
abstract A method for detecting a target nucleic acid, which method includes the steps (i) amplifying the target nucleic acid to obtain an amplification product using a polymerase, a first primer with or without a segment noncontiguous to a first priming sequence, and a second primer with or without a segment noncontiguous to a second priming sequence in the presence of an oligonucleotide which is incapable of acting as a primer for the polymerase, wherein the oligonucleotide has at least 5 consecutive nucleotides fully complementary to at least 5 consecutive nucleotides of the first primer; and (ii) detecting the presence of the target nucleic acid by monitoring amplification thereof. In particular, the figure illustrates the method wherein the primer contains a segment noncontiguous to its priming sequence.
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