http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0733063-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5bc5292a07e34d16eb1d033a16d3aec3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-59 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-59 |
| filingDate | 1994-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2003-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_019a453782cfcf702b440c43aea27a6d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fbdf4deb69c96c48e4573049ea63022 |
| publicationDate | 2003-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0733063-B1 |
| titleOfInvention | Compositions and kits for fluorescence polarization assay of large molecules |
| abstract | An improved oligopeptide composition for use in a fluorescent polarization immunoassay for a high molecular weight analyte is disclosed, along with a kit and a method using the composition. The composition comprises an oligopeptide selected by a screening procedure in which a plurality of different oligopeptides having respective amino acid sequences that represent sequential overlapping segments of the analyte amino acid sequence, and a fluorescent label bound thereto. The screening process further includes screening to select a composition which exhibits fluorescence enhancement upon binding the analyte. A preferred embodiment of the oligopeptide is one having an amino acid sequence which does not form internal disulfide bridges. Such a preferred oligopeptide will generally have no more than one cysteine residue. In a further preferred embodiment, the fluorescent label is tetramethylrhodamine or a cyanine dye. The kit may be packaged with instructions directing a user to prepare an assay solution containing the monoclonal antibody and the oligopeptide in certain respective concentrations. The composition, method and kit are constructed to detect nanomolar concentrations of the analyte. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102175846-A |
| priorityDate | 1993-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 76.