| abstract |
A method of discriminating nucleic acids by examining the presence or absence of variant genes in a nucleic acid, the ratio of normal genes to variant genes, or the identity among specified genes present in various samples, which method comprises amplifying a specified region of a target nucleic acid present in a specimen by the polymerase chain reaction using a primer bearing a detectable marker and a primer bearing a site capable of binding to a solid-phase carrier, conducting competitive hybridization by adding the resultant nucleic acid as a marker sample DNA in at least an equimolar amount to a non-marker standard DNA of which the identity with the sample DNA is to be discriminated, and then measuring the marker intensity of the product of hybridization. A testing set for discriminating nucleic acids according to the above method comprises a primer to be used for amplifying a target nucleic acid and comprising a primer bearing a detectable marker and a primer bearing a site capable of binding to a solid-phase carrier, and a non-marker standard DNA of which the identity with a gene amplified by the amplifying primer is to be discriminated. |