abstract |
PCT No. PCT/EP93/02054 Sec. 371 Date Feb. 7, 1995 Sec. 102(e) Date Feb. 7, 1995 PCT Filed Aug. 2, 1993 PCT Pub. No. WO94/03608 PCT Pub. Date Feb. 17, 1994Hypoglycosylated recombinant glucose oxidase with a molecular weight of ca. 68-80 kDa, a specific activity of ca. 200 U/mg unit of weight, a carbohydrate portion of ca. 12% which is obtainable by expression of a recombinant DNA containing the GOD gene in the N-glycosylation-defective yeast mutants DSM 7042, DSM 7338, DSM 7160 or DSM 7340 or allelic mutant strains, fermentation and isolation of the enzyme from the culture supernatant or the cells. |