| abstract |
A catalytically active, truncated human cholesterol 7α-hydroxylase (CYP7) is provided. The truncated human CYP7 can be made recombinantly and thus recovered in relatively large amounts. Vectors and host cells for recombinant expression are provided, as well as an antibody which specifically recognizes an epitope of catalytically active, truncated human CYP7. Also provided is a method for screening a compound for its effect on expression of non-truncated human CYP7, and a method for screening a compound for its effect on non-truncated human CYP7 enzyme activity. |