abstract |
PCT No. PCT/EP93/01661 Sec. 371 Date Mar. 28, 1994 Sec. 102(e) Date Mar. 28, 1994 PCT Filed Jun. 28, 1993 PCT Pub. No. WO94/03595 PCT Pub. Date Feb. 17, 1994The present invention is concerned with a genetically modified live herpesvirus vaccine of which the live herpesviruses are not shed into the environment by a vaccinated animal. This can he achieved by deleting a gene encoding a gD homolog, such as gp50 of pseudorabies virus, and propagating this deletion mutant in a cell line expressing the gene product. The reduced shedding characteristic of such a vaccine is particularly advantageous in a vector vaccine. The present invention also concerns a quadruple mutant pseudorabies virus vaccine, and a live cell bound vaccine containing gp50 mutant pseudorabies virus and cells which do not complement the mutation. |