| abstract |
The present invention provides a novel β1→6 N -acetylglucosaminyltransferase, which forms core 2 oligosaccharide structures in O -glycans, and a novel acceptor molecule, leukosialin, CD43, for core 2 β1→6 N -acetylglucosaminyltransferase activity. The amino acid sequences and nucleic acid sequences encoding these molecules, as well as active fragments thereof, also are disclosed. A method for isolating nucleic acid sequences encoding proteins having enzymatic activity is disclosed, using CHO cells that support replication of plasmid vectors having a polyoma virus origin of replication. A method to obtain a suitable cell line that expresses an acceptor molecule also is disclosed. |