http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0586922-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_71bd505b63d09e7643964d94eee551b0 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M23-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M27-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M25-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-02 |
filingDate | 1993-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b6f95e15b7d65413011458b0a686101 |
publicationDate | 1994-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0586922-A1 |
titleOfInvention | Device for cell culture |
abstract | A culture bottle-like culture vessel (1) for cell cultures is known which, forming a supply chamber, receives a nutrient medium in which at least one production chamber which receives the cell cultures is arranged and which is separated from the nutrient medium by a dialysis membrane (15) and with a gas supply and discharge into the supply chamber. In order to provide a culture vessel for the production of cell cultures with a high cell density, which can be produced inexpensively and is easy to handle and in which the risk of infections is reduced, it is proposed that the gas supply and discharge required and arising in the cell cultivation Gas permeable gas exchange membrane (8) is provided, which forms at least part of the outer wall of the culture vessel and the total area, material and thickness are selected so that the permeability to oxygen per 10⁷ cells is at least 0.01 mg / h. Methods for cell cultivation are also known in which a culture medium is filled with a nutrient medium which flows around a production chamber (15) which is closed on all sides and accommodates the production chamber (15) to be cultivated, and the culture vessel is set into a continuous rolling movement. In order to specify a method based on this, which is simple to carry out and with which high cell densities can be produced within a short time and at the same time high vitality, the production chamber is filled with the cell culture to be cultivated in such a way that it contains an air bubble (24), the volume of which Occupies 10% of the volume of the production chamber and the rolling movement is superimposed on a tumbling movement such that the air bubble is moved in the cell culture. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1027426-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03048296-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7541178-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0765933-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0765934-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0765933-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0765935-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0765934-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0765935-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1027425-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0789072-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0789072-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1027425-A1 |
priorityDate | 1992-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.