| abstract |
PCT No. PCT/DK92/00138 Sec. 371 Date Nov. 11, 1993 Sec. 102(e) Date Nov. 11, 1993 PCT Filed Apr. 30, 1992 PCT Pub. No. WO92/19729 PCT Pub. Date Nov. 12, 1992The invention relates to stabilized subtilisin proteases wherein one or more prolines is substituted for a native amino acid at stabilizing positions defined both by the range of the dihedral angles in the primary structure present at the substitution site and by selected characteristics of the protease secondary structure in the vicinity of the substitution site, to nucleotide sequences that encode the stabilized proteases, and to host organisms that contain the nucleotide sequences encoding the stabilized proteases. |