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filingDate 1984-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1994-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0581329-A1
titleOfInvention Method of isolating restriction fragment deletions in vibrio cholerae, and products thereof
abstract This invention relates to a method of isolating deletion mutants of Vibrio cholerae , wherein the deletion is predetermined by digestion with restriction endonucleases of known specificity. The deletions are inserted into the Vibrio cholerae chromosome by in vivo recombination between a plasmid carrying the desired deletion, with adjacent flanking sequences, and the Vibrio cholerae chromosome. The invention includes the isolation and characterization of a new Vibrio cholerae strain having a deletion in the tox gene, as defined by Acc I, Xba I, Cla I and/or restriction endonuclease sites.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0928831-A1
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