| abstract |
In order to enrich an inhibitor of the proliferation of endothelial cells extracted from tissues of vertebrate animals, the selected initial tissue is centrifuged, for example embryonic tissue or adult brain tissue of birds or mammals, the extract is homogenized of tissue, then it is placed on a cation exchanger and the substances which bind to the cation exchanger are subjected to fractionation. The active fractions are separated by chromatographic gel filtration and cleaned on a reverse phase of high-yield liquid chromatography. The inhibitor thus obtained is useful for treating pathological conditions which require the inhibition of angiogenesis, for example for treating tumors, rheumatoid arthritis, diabetic retinophaty and retrolental fibroplasia, as well as for regulating neovascularization during treatment. injuries. |