| abstract |
Determination of ammonia, α-amino acid, corresponding α-keto acid or ionic species thereof by reacting a specimen solution with a reagent containing 1) amino acid dehydrogenase, 2) A₁, 3) B₁, and 4) if necessary, other ingredient(s) required for forming the cycle shown in the drawing, and determining the amount of A₂ formed or the amount of B₁ consumed; and a composition containing the ingredients 1), 2), 3) and 4) for use in the above determination method. The method and the composition make it possible to determine a small amount of a specimen solution conveniently with high sensitivity and are useful in the field of clinical inspection and food testing. |