| abstract |
For the controlled, restricted proteolytic cleavage of proteins, in particular proenzymes, a treatment with proteases is carried out in the presence of a detergent or in the presence of a chaotropic substance, with the exception of coagulation-active salts. Enzymes or protein fragments are obtained. The method is explained in particular using an easy-to-carry out method for the targeted cleavage of prothrombin in order to obtain thrombin. |