Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_27baedcc4f0f8a0e589c39d66576d7f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2495cc3f476e4ccb34d4b064ce8605d9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dba21b44489a6bcfd61532dc9e273ece http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c8302d76c150e11ef8de84e6d471c539 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_259ec66e390aaaa5c4c4073b28ba6e74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_136609191f84a9deab0356e778f14611 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_900a1e36f5b58c31215b2985262e705d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8722cbe395cb92e6b1268694cdb82f79 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6439 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6408 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
filingDate |
1993-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
1998-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_014bb8c081e241e0faf93f372ed50d69 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be674c05e65c64f8b7a7f67143352b6e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3064a4b6d5faac1981103da70ffd2a3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0fcc84e2c7ca2dc7f9d437868797a764 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c79187d76e3c2ea746d76993810f1fad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f3906e32f5ef10b5c9864c1ea5d69f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0618ed7e367809f2da8a5077e2de5c96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_22a4d00de5c8f67349a928be8ab83b4f |
publicationDate |
1998-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-0563998-B1 |
titleOfInvention |
A process for detecting biomolecules, toxic substances, polymers and pharmaceutical substances by time-resolved laser spectroscopy |
abstract |
The invention relates to a method for the quantitative and qualitative optical detection of labelled molecules by time-resolved fluorescence spectroscopy. In the method, dyes or molecules labelled by one or more dye molecules (dye conjugates) are specifically coupled to biomolecules, toxic substances, polymers or pharmaceutical agents and characterised by the dye-specific fluorescence lifetime which is unaffected by the labelled molecule. The use of multiplex dyes (same absorption and emission behaviour but different fluorescence lifetimes) makes possible optimal characterisation, on the basis of their different fluorescence lifetimes, of various substances which are present in a mixture after specific labelling with multiplex dyes and with use of the same excitation light source and detection unit. Detection of the time-course of the fluorescence intensity (fluorescence lifetime) makes it possible to dispense with the filter technique hitherto used and to suppress temporarily the scattered light, which leads to an increase in sensitivity compared with the methods used at present. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10151688-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2449771-C2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9757725-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9062342-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9334528-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9914119-B2 |
priorityDate |
1992-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |