Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_49f04b1dc6b9fcc2d8b159c5ebee364b |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-145555 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
filingDate |
1992-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5dd65e4201768d883e06f0f104ccf807 |
publicationDate |
1993-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-0556509-A2 |
titleOfInvention |
Method for discriminating types of nucleic acid bases |
abstract |
This invention provides a method for discriminating four kinds of nucleic acid bases of DNA at high speed by utilizing chromophores intrinsic to DNA. The method comprises the steps of adding the sample to a polar glassy solvent; reducing a temperature the glassy solution; adding a (n,π*) quencher as a fluorescence intensifying reagent to the solution; irradiating an excitation light of UV laser beams thereto; measuring lifetimes of fluorescence from the sample; and discriminating the nucleic acid bases of DNA. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1104491-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6716394-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5528046-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1104491-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0682671-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0682671-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5739040-A |
priorityDate |
1991-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |