Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c7ad58d3c0b071069a8ee0cb8c8ba2e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6115b7f4561db088f26a575e9339fed0 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-101666 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-107497 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5094 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-487 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-49 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-27 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-80 |
filingDate |
1992-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
1998-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0901e74094b5aa42b98fb9120926c1a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_773b58b305eeee3d381e4025e53ecbe4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3505d2b9c3dcad544886a4f7114760ed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58a311d2715c21d4c1c5e75620280a3e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_18b22899f2108e56229abe3f8e1ff43e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67a567a8015f9de62c0b9fd83be6051e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b468bd1681d0f1e41031cf13bc1086e |
publicationDate |
1998-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-0545314-B1 |
titleOfInvention |
Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood |
abstract |
Methods for characterizing and distinguishing reticulocytes and mature red blood cells use reagent compositions which include organic cationic dyes for staining the reticulocytes in the blood sample and buffer solutions for maintaining a pH of about 6 to about 9. The dyes may be the blue absorption dyes Oxazine 750 or New Methylene Blue. When a zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes, and the reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered and absorbed by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration. One method also includes adjusting the measured absorption signals for pseudo-absorption. |
priorityDate |
1991-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |