| abstract |
Methods of removing cells in the form of cell pellets from samples of liquid milk, or cultures or extracts of other food materials, or other materials of biological origin. Cells concentrated in the pellet can be analyzed using various techniques to determine the relative number of cells, including standard Breed Smear analyzes or cell lysis followed by measurement of ATP. A nucleic amplification can be carried out, for example by enzymatic amplification of the genome, using the cell pellet directly, without having to isolate the nucleic acid from the cells. After the amplification, an analysis can be carried out determining an amplified nucleic acid segment indicating the presence of cells to be studied in the sample. Thus, the invention provides methods for obtaining cellular constituents from milk samples, and from cultures or extracts of other materials, including food materials, and making it possible to determine a relative contamination of the milk as well. than other materials by microorganisms. The invention also relates to equipment making it possible to implement its various methods. |