http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0537185-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6bd36eb0a121b1b54a68e9d31632d05b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-16005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48 |
| filingDate | 1991-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3c3ff65ae846fbbc4ce1d66ff2ebc77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc1a7bcf706121fc63a42279ac4b5f65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0feb1fbcbbb2fcb69609548861a32aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6abe5871e93b4c0e1fb22aa8243aacde |
| publicationDate | 1993-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0537185-A1 |
| titleOfInvention | An enzymatic process for the preparation of derivatives of growth hormone releasing factor and peptides useful as intermediates in the process |
| abstract | Derivatives of the growth hormone releasing factor GRF(1-29)NH2 and its analogs are prepared by reacting a substrate component of the formula GRF'-Met-Ser-X, wherein GRF' represents the initial sequence GRF(1-26) or its analogues, comprising fragments of GRF(n-26), in which n is between 1 and 8 and X represents an acyclic alpha-amino carboxylic acid residue having a side chain uncharged hydrophilic which exhibits at least the size of a methyl group, preferably Ala, Thr, Ser, Asn or Gln, with H-Arg-NH2 as the nucleophilic component, in the presence of an L-specific serine or thiolcarboxypeptidase enzyme obtained from yeast or of animal, plant or other microbial origin, preferably CPD-Y from yeast, in an aqueous solution or dispersion having a pH between 6 and 9 and, if necessary, coupling the desired fragment termination N (1-(n-1)) by chemical or enzymatic process. Also described are GRF-related peptides of the formula GRF"-Met-Ser-X, where GRF" represents the initial sequence GRF(1-26), in which 1 to 4 of the amino acid residues may be replaced or deleted, or their des-alpha-NH2 derivatives, X corresponding to the description above, and which constitute effective intermediate products. The method allows for selective transpeptidation, yielding satisfactory amounts of biologically effective GRF-related amides. |
| priorityDate | 1990-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 405.