| abstract |
A probe for the quantitative or qualitative determination of one or more rRNA or tRNA-containing procaryotic or eucaryotic target organism(s) infecting a human or animal body, or for determining a genetic condition occurring in a human or animal body is described, said probe comprising a labelled oligonucleotide complementary to a unique rRNA or tRNA sequence of a target organism or a group or target organisms that is to be determined, and not hybridizing significantly with the non-target organism(s), said probe having been prepared byn (i) selecting a target organism or a group of target organisms, and (ii) preparing a labelled oligonucleotide which is complementary to a unique RNA sequence of said target organism(s) by hybridization screening and/or selection from a known RNA sequence, and (iii) verifying that the oligonucleotide does not significantly hybridize to non-target organisms. |